[Investigation of the preventive effect of glucosamine, chondroitin alone and in combination on cartilage insult caused by quinolone in juvenile rats]

Osteoarthritis is considered the most common joint disease in the world, especially among the aged population. But it may affect the kids as a secondary type when kids were administered some groups of drugs such as quinolones which cause cartilage deformations and predispose to secondary type of osteoarthritis. This study aims to investigate the protective effect of glucosamine and chondroitin alone and in combination in the treatment of arthritis in Juvenile Rats. This study was applied on 5 groups of juvenile wistar rats, which divided as: normal group, control group, the group of GA, group of CS, the group of combination between GA+CS. At the end of the study all of rats were killed, then measurement of maximum extension of the right knee were performed, also histological and functional analysis of the articular cartilage of the knee were performed. Control group showed the degenerative changes as compared to the normal group, which is improved into the groups of GA, CS, which distinguished from the combination group. This study shows that GA and CS have protective effect able to opposite the destructive effect of quinolone on immature cartilages

Spectrophotomeric Determination of Vigabatrin and Gabapentin in Bulk, Dosage Forms and Urine After Derivatization with P-Dimethyl Amiocinam Aldehyde

A selective and sensitive analytical method was developed for the determination of the Anticonvulsants, [Vigabatrin [I] and gabapentin [H]] in bulk, dosage forms and urine. The method is based on the reaction of both substances I, II through their aliphatic amino groups with p-dimethyl amino cinamaldehyde in non-aqueous medium to give orange color derivatives. The orange color was measured spectrophotometrically at 473 and 470 nm as lamda cmax for I and 11, respectively. The absorbance concentration plots were rectilinear over the range 20-140 micro g/mL The method applied to determine of I and II in their tablets, the percentage recoveries +/- SD [n = 10] were 100.7 +/- 0.32 and 101.2 +/- 0.41, respectively. The proposed method was successfully applied to the determination of I and II in spiked urine. The percent Recoveries +/- SD [n = 10] were 98.78 +/- 0.39 and 98.92 +/- 0.46 from spiked urine samples. The optimum conditions for the reaction were carefully investigated and incorporated into the procedure. No interference was encountered from the co administrated drugs: Valporic acid, dipheny phenobarbital, carbamazepine, clonazepam, clopazam or cimetidine. A proposal of the reaction pathway was suggested the advantages of the proposed method over existing method was discussed